2. In Vitro Screening of Probiotic Potential
The identified microorganisms must undergo rigorous in vitro testing to confirm their probiotic properties before advancing to further stages. Key evaluation parameters include:
- Resistance to Gastric Juices: Ability to survive the acidic environment of the stomach.
- Pathogen Reduction: Capacity to inhibit harmful gut pathogens.
- Antimicrobial Activity: Effectiveness against specific pathogens.
- Adherence to Human Epithelial Cells: Ability to colonize and interact positively with the gut lining.
- Safety Assessment: Ensuring no production of toxins, harmful metabolites, or adverse side effects.