Microbiological Testing Methods For Food Products

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Microbiological Testing Methods For Food Products

  1. A specific medium used in microbiological laboratories to culture or grow various types of microorganisms in order to identify and detect them. A culture medium is often made up of various nutrients to promote microbial growth. Cultural procedures have always been the preferred tests for both ready-to-eat foods and fresh vegetables. However, immunoassay and PCR methods are now more often used than cultural methods, owing to recent advancements in newer testing technologies and validation studies demonstrating that culture methods are not appropriate for all food categories.
  2. Immunoassay is a microbiological test that uses an antibody or immunoglobulin to determine the concentration of a macromolecule in a solution. In many circumstances, the identified macromolecule from an immunoassay approach is a protein, and it is commonly referred to as a “analyte.” These analytes in biological liquids, such as urine or serum, are frequently analysed using immunoassay test methods for a variety of applications. ELISA (Enzyme-Linked ImmunoSorbent Assay, also known as a “pregnancy test” or “dipstick” procedure) is one of the most widely used immunoassays.
  3. Dr. Kary Mullis created PCR in 1983, and it is a relatively new and groundbreaking approach. Today, it is a widespread and frequently crucial technology in medical and biological research labs for a wide range of applications. A PCR test can detect DNA or RNA fragments that are believed to be unique to the target bacterium. PCR is a technique that uses the ability of DNA polymerase to make billions of copies of a specific DNA sequence.
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